A role for the protease falcipain 1 in host cell invasion by the human malaria parasite.

نویسندگان

  • Doron C Greenbaum
  • Amos Baruch
  • Munira Grainger
  • Zbynek Bozdech
  • Katlin F Medzihradszky
  • Juan Engel
  • Joseph DeRisi
  • Anthony A Holder
  • Matthew Bogyo
چکیده

Cysteine proteases of Plasmodium falciparum are required for survival of the malaria parasite, yet their specific cellular functions remain unclear. We used a chemical proteomic screen with a small-molecule probe to characterize the predominant cysteine proteases throughout the parasite life cycle. Only one protease, falcipain 1, was active during the invasive merozoite stage. Falcipain 1-specific inhibitors, identified by screening of chemical libraries, blocked parasite invasion of host erythrocytes, yet had no effect on normal parasite processes such as hemoglobin degradation. These results demonstrate a specific role for falcipain 1 in host cell invasion and establish a potential new target for antimalarial therapeutics.

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عنوان ژورنال:
  • Science

دوره 298 5600  شماره 

صفحات  -

تاریخ انتشار 2002